Whole animal imaging

Whole-animal imaging of neural activity

Cell-to-cell heterogeneity is important in many biological processes. Although genomic and transcriptomic approaches to detect this heterogeneity are available, measuring the proteomes of single cells presents a much greater challenge. Herr, Schaffer and colleagues describe a western blotting approach for single cells, allowing the multiplex detection of up to 11 proteins per cell for ~2,000 ce...

Automated Whole Animal Bio-Imaging Assay for Human Cancer Dissemination

A quantitative bio-imaging platform is developed for analysis of human cancer dissemination in a short-term vertebrate xenotransplantation assay. Six days after implantation of cancer cells in zebrafish embryos, automated imaging in 96 well plates coupled to image analysis algorithms quantifies spreading throughout the host. Findings in this model correlate with behavior in long-term rodent xen...

Ultramicroscopy – Imaging a Whole Animal or a Whole Brain with Micron Resolution

Drosophila is the species of choice for a large range of questions that can be addressed using molecular genetic approaches. Its small size and translucent body also makes it a most suitable species for whole-body imaging with the light microscope. Hans-Ulrich Dodt and co-workers have developed an " ultramicroscope " that allows fast fluo-rescence imaging of Drosophila for high-throughput pheno...

Whole animal physiology redux

EVEN THOUGH I RETIRED COMPLETELY in 1998 and vowed never to attend another meeting, I had to renege because it is such a great honor to be invited to give this lecture honoring my mentor, Julius Comroe. I thank the selection committee for considering me. Of the nine scientists chosen since this lecture was established, I may be the first unreconstructed, whole animal physiologist. Before I left...

Whole Animal Oxygen Consumption